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The prognostic value of testicular microlithiasis as an incidental finding for the risk of testicular malignancy in children and the adult population: A systematic review. On behalf of the EAU pediatric urology guidelines panel
Center for Pediatric, Adolescent and Reconstructive Urology, Medical Faculty Mannheim, University of Medical Center Mannheim, Heidelberg University, Mannheim, Germany
The exact correlation of testicular microlithiasis (TM) with benign and malignant conditions remains unknown, especially in the paediatric population. The potential association of TM with testicular malignancy in adulthood has led to controversy regarding management and follow-up.
Objective
To determine the prognostic importance of TM in children in correlation to the risk of testicular malignancy or infertility and compare the differences between the paediatric and adult population.
Study design
We performed a literature review of the Medline, Embase and Cochrane controlled trials databases until November 2020 according to the Preferred Reporting Items of Systematic Reviews and Meta-Analyses (PRISMA) Statement. Twenty-six publications were included in the analysis.
Results
During the follow-up of 595 children with TM only one patient with TM developed a testicular malignancy during puberty. In the other 594 no testicular malignancy was found, even in the presence of risk factors. In the adult population, an increased risk for testicular malignancy in the presence of TM was found in patients with history of cryptorchidism (6% vs 0%), testicular malignancy (22% vs 2%) or sub/infertility (11–23% vs 1.7%) compared to TM-free. The difference between paediatric and adult population might be explained by the short duration of follow-up, varying between six months and three years. With an average age at inclusion of 10 years and testicular malignancies are expected to develop from puberty on, testicular malignancies might not yet have developed.
Conclusion
TM is a common incidental finding that does not seem to be associated with testicular malignancy during childhood, but in the presence of risk factors is associated with testicular malignancy in the adult population. Routine monthly self-examination of the testes is recommended in children with contributing risk factors from puberty onwards. When TM is still present during transition to adulthood a more intensive follow-up could be considered.
The clinical significance of testicular microlithiasis (TM) remains unclear, thus posing a strategic problem for clinicians. Despite an increased incidence due to improved sensitivity and availability of ultrasound equipment, the natural history of TM is unknown. TM is defined as hyperechogenic foci in the testicular parenchyma, in different degrees of presence and diffusely spread throughout the testes, often found bilaterally [
]. The echogenic shadow typically seen in renal lithiasis or calcifications is lacking in TM.
The size of TM found on ultrasound is generally 1–2 mm and has been associated with generalized testicular dysgenesis. When TM is associated with a testicular tumour it is mostly seen around or within the tumour [
Incidentally detected non-palpable testicular tumours in adults at scrotal ultrasound: impact of radiological findings on management Radiologic review and recommendations of the ESUR scrotal imaging subcommittee.
]. The discrepancy between radiological and histological TM makes the interpretation for future implications difficult.
Although the EAU/ESPU guidelines do not recommend routine ultrasound for undescended or non-palpable testis, there are various reasons why ultrasound of the testis is performed in children. TM is therefore often found as an incidental finding without any accompanying risk factors. TM can also be found in the presence of testicular pathology, such as a testicular tumor or undescended testes. This difference in presentation might be of significance when considering clinical consequences.
In the adult population, a routine ultrasound of the testes is indicated for infertility and suspicion of a testicular mass. In adults, testicular TM has been associated with a significantly increased risk for testicular malignancy compared to men in whom TM was absent (risk ratio of 8.5, 95%CI 4.5–16.1) [
Tan et al Testicular microlithiasis predicts concurrent testicular germ cell tumors and intratubular germ cell neoplasia of unclassified type in adults: a meta-analysis and systematic review.
Xu et al The association between testicular microlithiasis and semen parameters in Chinese adult men with fertility intention: experience of 226 cases.
]. However, no direct causative association between TM and malignancy or fertility has ever been found. The incidence of TM might be increased in benign conditions such as Klinefelter's Syndrome, cryptorchidism, hypospadias and post trauma [
]. The question that arises therefore is; is there is a higher incidence of TM in these patient groups, or is it because they undergo more frequent imaging studies? The exact correlation of TM with both benign and malignant conditions remains unknown, especially in the paediatric population. The potential association of TM with testicular malignancy and infertility in adulthood has led to controversy regarding management and follow-up. It is not clear if data on adults can simply be extrapolated to children and adolescents.
The first aim of this systematic review (SR) is to determine the prognostic importance of the diagnosis of TM in children and correlate this finding with the risk of testicular malignancy or infertility. Subsequently we compare the differences between the paediatric and adult population. This comparison is based on a literature review of the SRs and meta-analyses available in adults on the correlation between TM and testicular malignancy as well as infertility. Finally, we aim to provide a guideline for clinicians on the interpretation of the incidental finding of the diagnosis of TM and its clinical consequences in children.
Evidence acquisition
This systematic review was performed according to the Preferred Reporting Items of Systematic Reviews and Meta-Analyses (PRISMA) Statement [
]. The a priori protocol is available at the PROSPERO database (CRD42020150898). The systematic review was structured into two sections; a systematic review about TM in the paediatric population and a literature search about TM in the adult population. The eligibility criteria and potential confounders (associated pathology such as cryptorchidism and testicular tumours) were identified by the European Association of Urology (EAU) Paediatric Urology guidelines panel.
Search strategy
For the first section we performed a literature search in the Medline, Embase and Cochrane controlled trials databases and clinicaltrial.gov for all relevant publications (no limitation for publication time and only English language) from 1946 until November 28, 2020. The patient group of interest were children under the age of 18 years who underwent a scrotal ultrasound for any indication and where TM was found in at least a proportion of the study population. Inclusion criteria included reporting of testicular tumours or infertility. Follow-up with any duration was included, but when no follow-up ultrasound was performed studies were excluded. Observational, interventional and prognostic studies were eligible for inclusion.
In the second part, we focussed on the available systematic reviews and meta-analyses about TM in the adult population. Again, a literature search was performed in the Medline, Embase and Cochrane controlled trials databases and clinicaltrial.gov for all relevant publications (no limitation for publication time and only English language) from 1946 until November 28, 2020. The patient group of interest were adults who underwent an ultrasound for any indication and where TM was found in at least part of the study population. The outcome of interest for the study was testicular malignancy or infertility. Systematic reviews and meta-analyses were eligible for inclusion.
For both sections two review authors have independently screened the titles and abstracts of identified records for eligibility. The full-text of all potentially eligible records were retrieved and screened independently by two review authors using a standardised form, linking together multiple records of the same study in the process. Any disagreements were resolved by discussion or by consulting a third review author.
Two review authors participated in the data extraction process. Study characteristics were extracted by one review author and a second review author checked data extractions for accuracy. Any disagreements have been resolved by discussion or by consulting a third review author.
Type of outcome measures
The primary outcome of the study was the prognostic value of TM (found on ultrasound) for testicular malignancy after 15 years of diagnosis. The secondary outcomes of interest were the prognostic value of TM for testicular malignancy after any follow-up, prognostic value of TM for infertility after any follow-up duration and presence of concurrent pathology, such as Down syndrome and McCune Albright Syndrome.
Risk of bias assessment
A risk of bias assessment was performed only for the included studies for the paediatric population. The risk of bias of each included study was assessed by two review authors working independently. Any disagreements were resolved by discussion or by consulting a third review author. Risk of bias was assessed by using the QUIPS tool as recommended by the Cochrane Prognosis Methods Group [
]. This includes the assessment of risk of bias across six domains informed by corresponding prompting items: study participation, study attrition, prognostic factor measurement, outcome measurement, study confounding (associated pathology) and statistical analysis reporting. All domains consist of several criteria of which the combined rating produces a classification of high, moderate, or low risk. The overall risk of bias was considered low if ≤ 2 domains were rated a moderate risk of bias and all others were rated a low risk of bias. The overall risk of bias was considered moderate if > 2 domains were rated a moderate risk of bias and all others were rated a low risk of bias. The overall risk of bias was considered high if ≥ 1 domain was rated a high risk of bias, irrespective of all other domains. The risk of bias assessment for the studies for the adult population was already performed within the included systematic reviews and meta-analyses.
Data analysis
Because of the lack of high quality evidence, we were unable to perform a meta-analysis of the data to assess the association between TM and testicular malignancy. We constructed a narrative synthesis to assess the extracted data for the paediatric population. A narrative synthesis was also constructed for the adult population. The differences in results between the two populations are summarized in text and tabulations.
Evidence synthesis
Quantity of evidence identified
The PRISMA flow diagram demonstrates the study selection process (Fig. 1). For the paediatric population a total of 210 titles and abstract were identified and 61 publications were retrieved for full-text screening. We found 15 studies eligible for inclusion with a total of 595 children for follow-up [
Long-term follow-up of testicular microlithiasis in children and adolescents: multicenter prospective cohort study of the Italian society of pediatric urology.
For the adult population a total of 38 titles and abstracts were identified and 11 publications were retrieved for full-screening. We found 7 systematic reviews eligible for inclusion, which included a total of 168 studies [
Fig. 2 demonstrates the risk of bias for the paediatric studies which also includes the confounder associated pathology. An overall high risk of bias was found for all studies.
Fig 2Risk of bias summary of the 15 included paediatric follow-up studies.
Characteristics of the included paediatric studies
The baseline characteristics of the included paediatric follow-up studies are summarized in Table 1. All of the included studies were observational studies, two prospective studies [
Long-term follow-up of testicular microlithiasis in children and adolescents: multicenter prospective cohort study of the Italian society of pediatric urology.
Diffuse: TM in >3 sections; Focal: TM in<3 sections
Increased: >20% increase in TM; Decreased: >20% decrease in TM; No change: <20% increase or decrease
Mean 79.1 mo (38.8 mo)
No standardized follow-up routine
Leenen et al (2002), 1996–1999
5
Mean 10.5 yrs (range 6–18 yrs)
Sixteen consecutive patients with characteristic TM who underwent US examination at our institution
NR
4 after orchiopexy; 4 UDT; 3 Palpable scrotal mass; 2 Peutz-Jehgers Syndrome; 1 Trauma; 1 pulmonary metastases; 1 ALL
Quantification of calcifications: Few (5–50) or multiple (>50) in a single plane. Distribution: focal clustering of foci in one-third only or in the periphery of the testicular parenchyma
NR
Mean 19 mo
NR
Kocaoğlu et al (2005), 1998–2004
9
Mean 9.2 yrs (range 3–16 yrs)
Children with TM at US between the recruitment period
NR
2 scrotal pain; 2 varicoceles; 1 bilateral UDT; 1 unilateral UDT; 1 Klinefelter Syndrome with bilateral orchiopexia; 1 trauma; 1 insufficient growth and development
Diffuse or focal and bilateral or unilateral, with or without accompanying nodules
NR
Mean 31 mo (9–62 mo)
Interval of 3–12 months in accordance with coexisting pathologies after the diagnosis of TM
Furness et al (1998), NR
23
Mean 12.3 yrs (range 6 months–21 yrs)
Incidentally discovered TM in childhood
Children with previous or concurrent testicular malignancy at time of diagnosis of testicular microlithiasis
Ultrasound findings include 1–3 mm, diffuse, punctate, nonshadowing, hyperechoic foci within testicular parenchyma
NR
Mean 27.6 mo (1mo-7yr)
Usually consisted of yearly ultrasound and physical examination
Dutra et al (2011), 2005–2010
11
Mean 7.5 yrs (range 1–15 yrs)
Children with UDT, retractile testis, hypotrophy of the testis and inguinal hernia were submitted to US
NR
5 UDT (3,93% of 127 pts); 4 retractile testis (14,8% of 27 pts); 1 testis hypotrophy (100% in 1 pt); 1 inguinal hernia (0,07% of 1349 pts).
Distributed hyperechogenic microliths <3 mm seen in a single ultrasound scan. The distribution of calcifications was diffuse or focal, uni or bilateral
NR
Range 6 mo–5 yrs
Annual follow-up with physical examinations and ultrasound evaluations
Multiple 1–3 mm echogenic foci within the parenchyma of the testis on US
NR
Mean 35 mo (8–67 mo)
Yearly US follow-up
Cooper et al (2014), 2003–2012
83
Mean 11.0 yrs (range 0.6–17.9 yrs)
All patients <18 yrs of age who had a scrotal US study and included the keywords “microlithiasis”, “microcalcifications” and “punctate calcifications”. At least one year follow-up.
Patients with testicular tumors who were diagnosed with TM only at retrospective review of the US studies not to bias the results
Classic TM > 5 microliths in 1 US image; Limited TM < 5 microliths in 1 US image. In CTM distribution was diffuse or clustered (localized in 1 area, patchy or nodular)
NR
Mean 4.2 yr (1–14.5yr)
NR
Volokhina et al (2014), 2000–2011
87
Mean 10.6 yrs
Symptomatic children referred for US exams for a very broad variety of reasons with classic testicular microlithiasis
NR
NR
Classic TM > 5 microliths in 1 US image; Limited TM < 5 microliths in 1 US image and were grouped together with children without TM
NR
Mean 265 days (9 days-4yr)
NR
Yesil et al (2016), 2008–2015
81
Mean 8.7 ± 4.1 yrs
Patients with TM who had undergone a scrotal US at least twice
Diffuse TM: microliths in >3 sections; Focal TM: microliths in <3 sections
NR
Mean 2.74 yrs (1.4yr)
Every 6 months, clinical and US evaluation + serum tumor markers (AFP and b-HCG)
Chiang et al (2011), 2002–2007
31
Median 11 yrs (range 4.7–14.8 yrs)
Clinical indication for scrotal US and pts with TM were included
NR
17 scrotal swelling or enlargement; 12 UDT; 10 hydrocele; 9 orchidalgia; 8 varicocele; 7 inguinal hernia; 7 torsion of hydatid cyst of Morgagni; 4 evaluation of testis size; 1 non-palpable testis
NR
NR
Mean 39.6 mo (0–128.6 mo)
NR
Goede et al (2010), 1990–2009
9
Mean 12.4 yrs (range 4.1–24.1 yrs)
Patients with acquired undescended (ascending) testis had follow-up
NR
Acquired undescended (ascending) testis
Classic TM: >5 echogenic foci 1–3 mm in either or both testes; Limited TM: <5 foci. TM was differentiated as diffusely scattered throughout the parenchyma or segmented
NR
Median 1.36 yrs (0–3.2yrs)
Full physical examination, additionally US was repeated to confirm the diagnosis.
Nishimura et al (2017), 2009–2016
56
Median age 11.3 mo (range 6.4–29.1 mo)
Patients with isolated congenital palpable UDT who underwent standard orchiopexy and preoperative testicular US evaluations
Patients with concomitant congenital anomalies, such as hypospadias, chromosomal anomaly, disorders of sexual development, endocrine disorders, and other symptoms related to UDT. Also patients who had failed orchiopexy, acquired UDT or >3 yrs at orchiopexy
Congenital undescended testis
Classic TM: >5 echogenic foci per field; Limited TM: <5 echogenic foci per field.
Progression: change in classification from LTM to CTM or when new TM was identified during follow-up in patients without TM on previous examination Improvement: change from CTM to LTM or disappearance of all TM on subsequent US
Median 24.9 mo (0.33–85.1 mo)
Serial US evaluations were performed before surgery, at 1 year after surgery, and different times at 2 years or later after surgery depending on the physicians preference
Riebel et al (2000), 1986–1996
68
Average 8 yrs 6 mo (range 3 yrs–13 yrs 6 mo)
Boys who had undergone surgical correction of UDT and who received a US examination
The definition for the diagnosis of TM varied between the studies. The most commonly used classifications were: Classic TM with ≥5 microliths in 1 US image and Limited TM < 5microliths in 1 image; Diffuse and Focal distribution; either bilateral or unilateral. TM was mostly found bilaterally and diffuse or classic distribution patterns were more common than limited or focal patterns.
Patients presented with different underlying pathology, including undescended testis, varicocele, inguinal hernias, scrotal pain or trauma, testicular masses or atrophy, Klinefelter Syndrome, Peutz-Jehgers Syndrome, McCune Albright Syndrome, Down Syndrome or no associated pathology.
Outcomes of the included studies
Paediatric population
The outcomes of the paediatric studies are presented in Table 2.
Table 2Outcomes of the paediatric follow-up studies.
Study (year)
N of tumors during follow-up
Tumor characteristics
N of concomitant tumors and TM
Type and distribution of TM
Change in TM during follow-up
Tumor markers
Testicular biopsy results
Incidence of TM according to associated pathology
Silveri et al (2011), 2002–2011
0/21
X
0/21
7 diffuse/14 focal 21 bilateral/0 unilateral
No change in TM pattern during FU
Normal
NR
NR
Marte et al (2017), 2008–2014
1 malignant/1 benign
1 seminoma (5yrs FU) in 17 year old/1 mature teratoma (3yrs FU) in 9 year old
1 seminoma in 17 year old
30 CTM/14 LTM 54 bilateral/27 unilateral
77/81 no change in TM pattern; 4/81 improved after surgery
Calcific density increased not significantly: 3,74%+-6,0% vs 3,06%+-4,38%. 14 testis were increased, 18 testes decreased and in 11 testis no change. Half of the pts with diffuse TM 10/20 compared to focal TM 4/23 were increased p = 0.049
NR
NR
NR
Leenen et al (2002), 1996–1999
0/5
X
1 germ cell tumor in 13 year old; 2 Sertoli-cell tumors (associated with Peuts-Jehgers syndrome)
15 diffuse/1 focal 11 bilateral/5 unilateral
4 no change and 1 reduction in size
NR
3 confirmed intratubular microcalcifications and 1 no detectable TM
NR
Kocaoğlu et al (2005), 1998–2004
0/9
X
0/9
7 diffuse/2 focal
NR
NR
NR
NR
Furness et al (1998), NR
0/23
X
Excluded; 1 benign Sertoli cell nodule
25 bilateral/1 unilateral
NR
15/15 showed normal tumour markers (AFP and b-HCG)
9 biopsies showed dystrophic calcifications without evidence of malignancy or abnormal seminiferous tubules.
1 less prominent TM; 4 unchanged; 2 lost to follow up
1/1 showed normal tumour markers (AFP and b-HCG)
NR
NR
Cooper et al (2014), 2003–2012
0/18
X
6 pts had a premalignant or benign tumor (5 pts <11 yrs and 1 pt 14.5 yrs); with predisposing conditions in five (83%) (2 cryptorchidism and 3 Peuts-Jeghers syndrome). Four malignant tumors were found, all in adolescent boys (range 16,2–17,8 yrs).
Testicular tumor with TM 10/83 (12%) vs. TM-free 10/3370 (0.3%)
Volokhina et al (2014), 2000–2011
0/9
X
1 mixed germ cell tumor in 16 year old
9 CTM
NR
NR
NR
NR
Yesil et al (2016), 2008–2015
0/78
X
0/78
56 diffuse/22 focal 45 bilateral/33 unilateral
2 decreased TM and 2 complete resolution of TM
b-HCG were within normal limits for all patients; AFP was slightly elevated in 7 patients (8,97%), all patients exhibited normal AFP levels upon follow-up.
6 (7,7%) biopsies were performed: 1 dermoid cyst, others normal testicular tissue or fetal arrest.
Prognostic value of the diagnosis of TM for testicular malignancy and the correlation or risk of testicular malignancy
Our primary outcome of interest was if the diagnosis of TM would be associated with testicular malignancy, post-pubertal, at 15 years follow-up, however, none of the studies had a follow-up exceeding 11 years.
Of the fifteen included studies with a total of 595 patients with TM only one study reported the development of 1 testicular malignancy during follow-up. This patient, aged 17 years, was diagnosed with a seminoma after 5 years of follow-up of bilateral TM. No other associated risk factors were reported in this patient.
From these 15 studies with 595 patients, only 20 testes demonstrated an increased TM pattern and 33 testes a decreased pattern or resolution of TM.
The presence of a concomitant testicular tumour and TM was reported in four studies [
Long-term follow-up of testicular microlithiasis in children and adolescents: multicenter prospective cohort study of the Italian society of pediatric urology.
]. This included seven germ cell tumours in boys all older than 13 years. In the five pre-adolescent boys only benign or premalignant tumours were reported, of these four had associated risk factors, i.e. cryptorchidism and Peutz-Jeghers Syndrome.
Four studies reported the evaluation of tumour markers [
Long-term follow-up of testicular microlithiasis in children and adolescents: multicenter prospective cohort study of the Italian society of pediatric urology.
Long-term follow-up of testicular microlithiasis in children and adolescents: multicenter prospective cohort study of the Italian society of pediatric urology.
]. In children with Down Syndrome the prevalence of TM ranged from 22.8 to 36% vs 0–7% in children without Down Syndrome. During the follow-up one patient with Down Syndrome presented with a Leydig cell tumour, he also had concomitant cryptorchidism. Follow-up of patients without Down Syndrome was not performed.
One study described the prevalence of TM in patients with McCune Albright Syndrome [
]. A prevalence of 24% of TM was reported. Of the 54 patients 16 presented with concomitant testicular tumours, 11 Leydig cell hyperplasia, one Leydig cell and one Sertoli cell intraepithelial neoplasia, one seminoma and one embryonal carcinoma. During follow-up no testicular malignancies were described.
Adult population
The outcomes of the adult studies are presented in Table 3. For the adult population the included studies were four systematic reviews [
Tan et al Testicular microlithiasis predicts concurrent testicular germ cell tumors and intratubular germ cell neoplasia of unclassified type in adults: a meta-analysis and systematic review.
The systematic reviews and meta-analyses sub-divided the adult population into seven groups: asymptomatic, symptomatic, cryptorchidism, sub/infertility, unspecified, with testicular tumour, with a positive family history and a specific prospective cohort.
Factors not associated with an increased risk for testicular malignancy in patients with TM
Patients who were asymptomatic (n = 3982) or who had a positive family history (n = 217) with TM did not show an increased risk for the development of testicular tumours [
Tan et al Testicular microlithiasis predicts concurrent testicular germ cell tumors and intratubular germ cell neoplasia of unclassified type in adults: a meta-analysis and systematic review.
]. The prevalence of TM was higher for the population with a positive family history compared to asymptomatic patients, however, these data are based on a single study.
Factors associated with an increased risk for testicular malignancy in patients with TM
In the symptomatic patient group (n = 22,763) an increased risk for testicular malignancy was found when TM was present [
Tan et al Testicular microlithiasis predicts concurrent testicular germ cell tumors and intratubular germ cell neoplasia of unclassified type in adults: a meta-analysis and systematic review.
]. Symptomatic was defined as testicular pain, testicular edema or increased testis volume. The risk was increased with a RR14.2 for the group with TM in one systematic review and a significant difference of 11.2% with TM vs 1% TM-free in another systematic review. Prevalence of TM ranged from 0.6 to 18.1%.
In adult men with a history of cryptorchidism (n = 1455), an increased risk for testicular malignancy of 6% was found in patients presenting with the diagnosis of TM compared to 0% in the TM-free population [
]. The prevalence of TM was reported with a wide range from 2.8 to 36.5%.
The sub/infertility group (n = 9295) also demonstrated a higher risk for testicular tumour when TM was present on ultrasound [7,31,32,34,35]. The risk of tumour in the group with TM was reported to be RR 15.6, OR 18.6 and with significant differences of 10.9–22.6% in the TM group vs 1.6–1.7% in the TM-free group. Prevalence was reported to be between 0.9 and 20.1%.
Patients that had a history of testicular malignancy with TM (n = 156) showed an increased risk for testicular tumour of 22% vs 2% in the TM-free group [
Tan et al Testicular microlithiasis predicts concurrent testicular germ cell tumors and intratubular germ cell neoplasia of unclassified type in adults: a meta-analysis and systematic review.
Tan et al Testicular microlithiasis predicts concurrent testicular germ cell tumors and intratubular germ cell neoplasia of unclassified type in adults: a meta-analysis and systematic review.
]. The incidence of testicular malignancy during follow-up in patients with TM ranged between 1 and 7%. Only two patients did not have known risk factors for testicular tumour, while the other patients had infertility, cryptorchidism, testicular tumour of testicular atrophy.
Discussion
Principal findings
With this systematic review we present the results of follow-up in the largest group of paediatric patients, 595 form 15 studies, with the diagnosis of TM. During follow-up only one patient with TM developed a testicular malignancy and this was during puberty. In the other 594 patients no testicular malignancy was found, even in the presence of other risk factors for testicular malignancy such as cryptorchidism. However, it is important to emphasize that the follow-up duration mostly varied between six months and three years, but never exceeded 11 years. Given that the average age was about 10 years and testicular malignancies are expected to develop from puberty on, it is very well conceivable that testicular malignancies had not yet developed.
It was also shown that there is no additional value to determine tumour markers or perform testicular biopsies in children with TM, since this did not have any clinical consequences.
TM was described according to different classifications; classic vs limited and diffuse vs focal. This did not correlate with change in TM during follow-up or association with testicular malignancy. There seems to be no preferred classification system.
In the adult population, an increased risk of testicular malignancy in the presence of TM was observed for the various subgroups, specifically patients with a history of cryptorchidism, sub/infertility and a history of testicular malignancy. While patients with TM that were asymptomatic or had a family history of testicular malignancy were not at risk.
The fact that patients with TM and additional risk factors show an increased risk for testicular malignancies during adulthood confirms the hypothesis that the follow-up of paediatric patients with TM might have been too short.
A systematic review and meta-analysis was published in 2019 by Yu et al. [
Incidence characteristics of testicular microlithiasis and its association with risk of primary testicular tumors in children: a systematic review and meta-analysis.
], also investigating the association between TM and testicular tumours in children. They included 10 follow-up studies with 296 children. They report four tumours during follow-up, however, they included benign tumours and concurrent testicular tumours at diagnosis of TM. In our analysis we have separated the concomitant diagnosis testicular tumour with TM, since this does not demonstrate what happens with TM as an incidental finding. In addition, we were able to include 5 more studies and include 199 more children to further strengthen the results. Also, a systematic comparison to the adult literature has now been performed.
Implications for clinical practice
Based on the current literature we would recommend that in asymptomatic children and without risk factors; where TM is incidentally found on ultrasound this warrants no further investigation or follow-up.
In children where TM is found in the presence of risk factors, such as cryptorchidism, monthly self-examination from puberty is advised without additional routine ultrasound follow-up. The available data do not support the need for earlier self-examination, which would be difficult in the paediatric population.
An important moment arises during the transition phase from paediatric to adult urology, especially for patients that present with sub/infertility issues and a history of TM and additional risk factors highlighted in this review. Based on the EAU guidelines Sexual and Reproductive Health this is a group of patients in whom the option of yearly US follow-up and even testicular biopsies has to be considered [
]. This specific group of patients might benefit from this more intensive follow-up and a referral to the adult urologist might be indicated for children with TM and additional risk factors when they reach the age of 18 years.
Further research
It is imperative that studies with a long-term follow-up of children diagnosed with TM with and without risk factors should be conducted, specifically follow-up exceeding puberty. One could propose a study were children with TM are called back for medical history and a current ultrasound investigation at age 25 or 30 years. This will hopefully answer the still remaining questions regarding TM:
-
Is TM found during childhood the same entity as TM found in adults with a concomitant pathology?
-
What is the origin of TM? Could it be the result of intratesticular trauma, or obstruction or inflammation? Or is it indeed a precursor for testicular malignancy?
-
Is there a correlation between TM and sub/infertility?
Future research should focus on prospective studies in which the role of possible contributing risk factors can be investigated and the clinical consequence of TM is more elucidated.
Limitations and strengths of the study
In this systematic review several strengths and limitations need to be addressed.
First, in the paediatric population only observational studies could be included and only two of these studies were prospectively conducted. Also, patients with various associated pathologies were grouped together and not controlled for, resulting in an increased risk of bias.
The second limitation of the systematic review is the heterogeneity of the data in the adults. The prevalence of TM is reported with a wide range for the different patient subgroups, indicating that the original studies included in the systematic reviews have a high risk of bias.
The third main limitation of the systematic review is that the reported follow-up did not exceed puberty in most studies looking at TM in children, even when risk factors were present. Testicular malignancies are expected to occur from puberty on and it is therefore feasible that testicular malignancies may have occurred after completion of the study. Especially, if the data from the paediatric population are compared to the adult population where this increased risk for testicular malignancy has been shown for patients with additional risk factors.
This immediately highlights one of the strengths of this study. A systematic approach was followed to collect the data of both children and adults and a direct comparison could be made between these two populations.
One of the other strengths of this study is that it represents the largest collection of follow-up data in children and thereby best reflects the available evidence in the literature.
Conclusion
TM is a relatively common incidental finding at testicular ultrasound. In the paediatric population TM does not seem to be associated with testicular malignancy. In the adult population TM in combination with a history of cryptorchidism, sub/infertility or a previous history of testicular tumours is associated with an increased risk for testicular malignancy. Routine monthly self-examination of the testes is only recommended in children with contributing risk factors from puberty onwards. When TM is still present with accompanying risk factors during transition to adulthood a more intensive follow-up with ultrasound and even biopsy could be considered.
Conflict of interest
None.
Acknowledgements
This systematic review was performed under the auspices of the European Association of Urology and the European Society for Pediatric Urology, Pediatric Urology Guidelines Panel.
References
Miller
et al.
Testicular microlithiasis: a benign condition with a malignant association.
Incidentally detected non-palpable testicular tumours in adults at scrotal ultrasound: impact of radiological findings on management Radiologic review and recommendations of the ESUR scrotal imaging subcommittee.
Testicular microlithiasis predicts concurrent testicular germ cell tumors and intratubular germ cell neoplasia of unclassified type in adults: a meta-analysis and systematic review.
Long-term follow-up of testicular microlithiasis in children and adolescents: multicenter prospective cohort study of the Italian society of pediatric urology.
Incidence characteristics of testicular microlithiasis and its association with risk of primary testicular tumors in children: a systematic review and meta-analysis.
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