Summary
Aquaporin 1 (AQP1) is the archetype of all aquaporins and involved in rapid cellular
water fluxes and cell volume regulation.
An objective
This study was conducted for the investigation of AQP1 expression in normal testicular
tissues and those with I/R injury in a rat model.
Study design
A TT rat model was established using male Wister rats (4 weeks old, 180–220 g), and
AQP1 distribution in the testicular tissues was detected by immunohistochemistry.
The wet/dry (W/D) weight ratios of the testes were determined at 12 h, 24 h, 36 h,
48 h, or 5 days after the establishment of the TT model. At each time point, pathological
sections were prepared and the mRNA and protein expression levels of AQP1 were determined
by RT-qPCR and Western blotting, respectively.
Results
Immunohistochemical staining indicated that AQP1 distributes in testicular vascular
endothelial cells and interstitial connective tissues. The testicular edema was observed
12 and 24 h after TT, as indicated by the increase in wet/dry weight ratio and pathological
changes, such as enlarged testicular interstitium, atrophy of spermatogenic tubules,
and epineurium tubule exfoliation. Increase in the expression levels of Aqp1 mRNA
and AQP1 protein levels peaked at 24 h. Edema was alleviated at 36 and 48 h, as manifested
by the gradual thinning of the spermatogenic tubules epithelium with narrowed interstitium
and weakened inflammatory cell infiltration. Meanwhile, the mRNA and protein levels
of AQP1 dramatically decreased. At 5 days after TT, edema was nearly absent, and the
mRNA and protein levels of AQP1 were restored to basal levels.
Discussion
Testicular torsion increases AQP1 expression and W/D ratios in testis tissues. The
upregulation of AQP1 expression and decline in AQP1 level are consistent to the development
and alleviation of edema in testis tissues that underwent testicular torsion.
Conclusion
Graphical Abstract
Keywords
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Article info
Publication history
Published online: December 08, 2020
Accepted:
December 3,
2020
Received in revised form:
November 1,
2020
Received:
July 28,
2020
Identification
Copyright
© 2020 Journal of Pediatric Urology Company. Published by Elsevier Ltd. All rights reserved.
