Rantes –403g>a Functional Polymorphism and Upper Urinary Tract Infections in Children

Artifoni, Lina; Benetti, Elisa; Stefanic, Alejandra; Negrisolo, Susanna; Montini, Giovanni; Zucchetta, Pietro; Rigamonti, Waifro; da Dalt, Liviana; Murer, Luisa

doi:10.1016/j.jpurol.2009.02.010

« Previous Next »Journal of Pediatric Urology
Volume 5, Supplement 1 , Page S21, April 2009

Rantes –403g>a Functional Polymorphism and Upper Urinary Tract Infections in Children

Lina Artifoni
- Laboratory of Immunopathology and Molecular Biology of the Kidney, Department of Pediatrics “Salus Pueri”, University of Padua, Padua, ITALY
Elisa Benetti
- Pediatric Nephrology, Dialysis and Transplant Unit, Department of Pediatrics, Universisty of Padua, Padua, ITALY
Alejandra Stefanic
- Laboratory of Immunopathology and Molecular Biology of the Kidney, Department of Pediatrics “Salus Pueri”, University of Padua, Padua, ITALY
Susanna Negrisolo
- Laboratory of Immunopathology and Molecular Biology of the Kidney, Department of Pediatrics “Salus Pueri”, University of Padua, Padua, ITALY
Giovanni Montini
- Nephrology, Dialysis and Transplant Unit, Department of Pediatrics “Salus Pueri”, University of Padua, Padua, ITALY
Pietro Zucchetta
- Nuclear Medicine Service 1, University of Padua, Padua, ITALY
Waifro Rigamonti
- Urology Operative Unit, Institute of Urology, University of Padua, Padua, ITALY
Liviana da Dalt
- Department of Pediatrics “Salus Pueri”, University of Padua, Padua, ITALY
Luisa Murer
- Nephrology, Dialysis and Transplant Unit, Department of Pediatrics “Salus Pueri”, University of Padua, Padua, ITALY

# B01-7 (PP)

Introduction

Chemokines play a key-role in leukocytes recruitment in the site of inflammation, through the interaction with trans-membrane receptors. Genes that codify these chemokines and their receptors may present polymorphisms, which influence the proteins' level of expression and/or function. We have recently described an increased susceptibility to the development of acute pyelonephritis in children with interleukin 8 –251A>T polymorphism, presenting the first episode of upper urinary tract infection. Functional polymorphism of RANTES chemokine and its receptor CCR5 have been also identified.

Material and Methods

We carried out an association study of RANTES-403G>A and CCR5Δ32 in 273 children with the first episode of upper urinary tract infection (UTI). Control population was represented by 326 blood umbilical cord DNAs coming from the same geographic area.

Results

Statistical analysis showed that RANTES-403G>A phenotypic frequencies in the UTI group significantly differed from those of the control population (p=0,007). A significant difference persisted when the genotype AA+GA was compared with the GG one (p=0,002). Furthermore, comparing the frequencies observed in acute dimercaptosuccinic acid scan (DMSA)-positive and DMSA-negative patient with those observed in controls, a significant difference did emerge (p=0,023 and p=0,008 respectively). In addition, RANTES-403G>A polymorphism frequency was significantly different in patients with vesico-ureteric reflux compared with the control population (p=0,020). No difference were observed in the genotype distribution of CCR5Δ32 polymorphism.